eSTAT3 Knockout cell line (Vero)

eSTAT3 Knockout cell line (Vero) Order

Catalog#

YKO-R045

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

eSTAT3 Knockout cell line (Vero)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Green monkey

Gene Name

STAT3

Gene ID

103243410

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

Target region

Knockout region: Exon 7 (based on transcript STAT3-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STAT3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Vero cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STAT3 gene knockout eSTAT3 Knockout cell line (Vero) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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eSTAT3 Knockout cell line (Vero)

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